My research focuses on the role of dysfunctional microtubule nucleation in breast cancer metastasis. Microtubule nucleation in mammalian cells classically occurs at the centrosome, the microtubule organizing center (MTOC) in mammalian cells. The centrosome is an organelle made up of several proteins necessary for the initiation of microtubule nucleation to occur, such as gamma-tubulin and gamma-tubulin complex proteins (GCPs). These proteins make up the gamma-tubulin ring complex (gamma-TuRC), which is thought to be the main complex involved in microtubule nucleation. Recently we have found evidence that dysregulation of these proteins could have a significant role in the ability for reattachment to the endothelium for circulating breast tumor cells.

Immunofluorescence, fluorescence microscopy, confocal microscopy, live cell imaging, 3D cell culture/co-culture, molecular biology

BA, Johns Hopkins University, 2000, Natural Sciences/Public Health

MS, Johns Hopkins University, 2005, Biotechnology

 

I received my Bachelor of Arts degree from the Johns Hopkins University in 2000 in Public Health. After graduation, I worked at the National Cancer Institute at Frederick (NCI-Frederick) in the Image Analysis Laboratory where I acquired expertise in optical microscopy and image analysis techniques. During my time at NCI-Frederick, I also acquired a Master of Science in Biotechnology degree from the Johns Hopkins University.

Currently, I work in the laboratory of Dr. Stuart Martin. The focus of the research in the lab is studying the effects of cytoskeletal abnormalities in the progression of breast cancer metastasis (see "My PI" link below for more information).

1. Rebecca A. Whipple, Eric M. Balzer, Edward H. Cho, Michael A. Matrone, Jennifer R. Yoon, and Stuart S. Martin, Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cell lines, Cancer Res. (Submitted)

 

2. Dalit Barkan, Hynda Kleinman, Justin Simmons, Holly Asmussen, Anil K. Kamaraju, Mark Hoenorhoff, Zi-yao Liu, Sylvain V. Costes, Edward H. Cho, Stephen Lockett, Chand Khanna, Ann F. Chambers, and Jeffery E. Green, Transition  from Tumor Cell Dormancy to Metastatic Growth is Dependent on the Extracellular Matrixand Cytoskeletal Dynamics, (Submitted)

 

3. Changcheng Song, Zhen Xiao, Kunio Nagashima, Chou-Chi H. Li, Stephen J. Lockett, Ren-Ming Dai, Edward H. Cho, Thomas P. Conrads, Timothy D. Veenstra, Nancy H. Colburn, Qing Wang and Ji Ming Wang, The heavy metal cadmium induces valosin-containing protein (VCP)-mediated aggresome formation, Toxicol Appl Pharmacol. 2008 Jan 8 [Epub ahead of print]

 

4. Satinder S. Rawat, C. Zimmerman, B. T. Johnson, Edward H. Cho, Stephen J. Lockett, Robert Blumenthal and Anu Puri, Restricted Lateral Mobility of Plasma Membrane CD4 Impairs HIV-1 Envelope Glycoprotein Mediated Fusion, Mol Membr Biol. 2008 Jan-Feb;25(1):83-94

 

5. David R. Hodge, Edward Cho, Terry D. Copeland, Tad Guszczynski, Eric Yang, Arun K. Seth, and William L. Farrar, IL-6 Enhances the Nuclear Translocation of DNA Cytosine-5-Methyltransferase 1 (DNMT1) via Phosphorylation of the Nuclear Localization Sequence by the AKT Kinase, Cancer Genomics Proteomics. 2007 Nov-Dec;4(6):387-98

 

6. Pablo Iribarren, Keqiang Chen, Wanghua Gong, Edward H. Cho, Stephen Lockett, Badarch Uranchimeg, and Ji Ming Wang, Interleukin 10 and TNF-{alpha} synergistically enhance the expression of the G protein-coupled formylpeptide receptor 2 in microglia, Neurobiol. Dis. 2007 Jul;27(1):90-98. Epub 2007 May 5

 

7. Catherine M. Finnegan, Satinder S. Rawat, Edward H. Cho, Danielle L. Guiffre, Stephen Lockett, Alfred H. Merrill, Jr., and Robert Blumenthal, Sphingomyelinase Restricts the Lateral Diffusion of CD4 and Inhibits HIV Fusion, J. Virol. 2007 May;81(10):5294-5304. Epub 2007 Mar 7

 

8. Keqiang Chen, Pablo Iribarren, Jian Huang, Lingzhi Zhang, Wanghua Gong, Edward H. Cho, Stephen Lockett, Nancy M. Dunlop, and Ji Ming Wang, Induction of the Formyl Peptide Receptor 2 in Microglia by IFN-{gamma} and Synergy with CD40 Ligand, J. Immunol. 2007 Feb 1;178(3):1759-66

 

9. Edward H. Cho, Stephen J. Lockett, Calibration and standardization of the emission light path of confocal microscopes, J. Microsc. 2006 Jul;223(Pt 1):15-25

 

10. Keqiang Chen, Pablo Iribarren, Jinyue Hu, Jianhong Chen, Wanghua Gong, Edward H. Cho, Stephen Lockett, Nancy M. Dunlop, Ji Ming Wang, Activation of toll-like receptor 2 on microglia promotes cell up-take of Alzheimer's disease-associated amyloid beta peptide, J Biol Chem. 2006 Feb 10;281(6):3651-9. Epub 2005 Dec 9.

 

11. Pablo Iribarren, Keqiang Chen, Jinyue Hu, Wanghua Gong, Edward H. Cho, Stephen Lockett, Badarch Uranchimeg, Ji Ming Wang, CpG-containing oligodeoxynucleotide promotes microglial cell uptake of amyloid beta 1-42 peptide by up-regulating the expression of the G-protein-coupled receptor mFPR2, FASEB J. 2005 Dec;19(14):2032-4. Epub 2005 Oct 11

 

12. David R. Hodge, Benjamin Peng, Celine Pompeia, Suneetha Thomas, Edward Cho, Peter A. Clausen, Victor E. Marquez, William L. Farrar, Epigenetic Silencing of Manganese Superoxide Dismutase (SOD-2) in KAS 6/1 Human Multiple Myeloma Cells Increases Cell Proliferation, Cancer Bio. & Ther. 2005 May;4(5):585-592 Epub 2005 May 5

 

13. Delphine Muriaux, Sylvain Costes, Kunio Nagashima, Jane Mirro, Ed Cho, Stephen Lockett, Alan Rein, Role of Murine Leukemia Virus Nucleocapsid Protein in Virus Assembly, J. Virol.  2004 Nov;78(22):12378-12385

 

14. Dirk Daelemans, Sylvain V. Costes, Edward H. Cho, Rebecca A. Erwin-Cohen, Stephen Lockett, George N. Pavlakis, In vivo HIV-1 Rev multimerization in the nucleolus and cytoplasm by fluorescence resonance energy transfer, J. Biol. Chem. 2004 Nov;279(48):50167-50175

 

15. Ali Saleh, Gareth E. Davies, Veronique Pascal, Paul W. Wright, Deborah L. Hodge, Edward H. Cho, Stephen J. Lockett, Mehrnoosh Abshari, Stephen K. Anderson, Identification of Probabilistic Transcriptional Switches in the Ly49 Gene Cluster: A Eukaryotic Mechanism for Selective Gene Activation, Immunity 2004 Jul;21:55-66

 

16. Ronghua Sun, Pablo Iribarren, Ning Zhang, Ye Zhou, Wanghua Gong, Edward H. Cho, Stephen Lockett, Oleg Chertov, Filip Bednar, Thomas J. Rogers, Joost J. Oppenheim, Ji Ming Wang, Identification of Neutrophil Granule Protein Cathepsin G as a Novel Chemotactic Agonist for the G Protein-Coupled Formyl Peptide Receptor FPR, J. Immuno. 2004 Jul;173(1):428-436

 

17. Sylvain V. Costes, Dirk Daelemans, Edward H. Cho, Zachary Dobbin, George Pavlakis, Stephen Lockett, Automatic and quantitative measurement of protein-protein co-localization in live cells, Biophys. J. 2004 Jun;86(6):3993-4003

 

18. A. Valance Washington, Rebecca L. Schubert, Laura Quigley, Theresa Disipio, Robert Feltz, Edward H. Cho, and Daniel W. McVicar, A TREM Family Member, TLT-1, Is Found Exclusively in the {alpha}-granules of Megakaryocytes and Platelets, Blood 2004 Aug;104(4):1042-1047 Prepublished online Apr 20, 2004

 

19. Qingsheng Yan, Edward Cho, Stephen Lockett, Kathrin Muegge, Association of Lsh with heterochromatin requires intact higher order heterochromatin structure, Mol Cell Biol. 2003 Dec;23(23):8416-28

 

20. Tao Fan, Qingsheng Yan, Jiaqiang Huang, Sharon Austin, Edward Cho, Doug Ferris, Kathrin Muegge, Lsh-deficient murine embryonal fibroblasts show reduced proliferation with signs of abnormal mitosis, Cancer Res. 2003 Aug 1;63(15):4677-83

 

21. Sylvain Costes, Olga Slobodskaya, Edward Cho, Maria Tsopanomichalou, George N. Pavlakis, Stephen Lockett, FRAP model to determine the bi-directional transport rate of GFP across the nuclear membrane and the mobile fraction in the cytoplasm and nucleus, Proceedings SPIE 2002 4622:32-39